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使用平板扫描仪,以收集拟南芥根向地反应的高分辨率时间失效图片
JoVE Journal
Biology
Author Produced
This content is Free Access.
JoVE Journal
Biology
Using Flatbed Scanners to Collect High-resolution Time-lapsed Images of the Arabidopsis Root Gravitropic Response
Please note that all translations are automatically generated.
Click here for the English version.
使用平板扫描仪,以收集拟南芥根向地反应的高分辨率时间失效图片
DOI:
10.3791/50878-v
•
08:25 min
•
January 25, 2014
•
Halie C Smith*
1
,
Devon J Niewohner*
1
,
Grant D Dewey*
1
,
Autumn M Longo*
1
,
Tracy L Guy
,
Bradley R Higgins
,
Sarah B Daehling
,
Sarah C. Genrich
,
Christopher D Wentworth
,
Tessa L Durham Brooks
1
Department of Biology
,
Doane College
,
2
Department of Physics
,
Doane College
Chapters
00:00
Title
00:52
Prepare Plates for Scanning
01:50
Set up Folders and Set Timers
03:10
Turn on Scanners and Prepare Settings
05:36
Place Plates in Scanners
07:12
Representative Results
07:59
Conclusion
Summary
Automatic Translation
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Automatic Translation
这个协议描述了用于快速收集拟南芥幼苗用市售的平板式扫描仪响应重力刺激的图像的处理。该方法允许对廉价,高容量的高清晰度图像适合用于下游分析算法捕获。
Tags
Flatbed Scanners
Time-lapsed Images
Arabidopsis
Root Gravitropic Response
High-resolution
High-throughput
Image Capture
Image Analysis
Phenotypes
Gene Function
Seedling Root
Gravity Vector
Corrective Growth
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