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Optogenetically लक्षित अग्रमस्तिष्क सर्किट की लेजर स्कैनिंग photostimulation
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Laser-scanning Photostimulation of Optogenetically Targeted Forebrain Circuits
DOI:

07:43 min

December 27, 2013

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Chapters

  • 00:05Title
  • 01:29Surgery and Injections
  • 01:52Slice Preparation
  • 02:37Physiological Recording with Laser-scanning Photostimulation
  • 04:22Fixed Slice Imaging
  • 05:16Channelrhodopsin Expression in V1-V2 and S1-VP Projections
  • 07:07Conclusion

Summary

Automatic Translation

We describe a method for characterizing the functional topography and synaptic properties of forebrain circuits using an optogenetic approach to photostimulate neuronal populations in vitro.

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