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培養された膀胱上皮を越えるヒト好中球の移行の定量的評価
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Quantitative Assessment of Human Neutrophil Migration Across a Cultured Bladder Epithelium
DOI:

11:20 min

November 07, 2013

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Chapters

  • 00:05Title
  • 01:27Seeding and Growing 5637 Cells on Permeable Supports |
  • 03:46Isolation of Human Neutrophils from Peripheral Blood
  • 06:22Transuroepithelial Neutrophil Migration Assay
  • 09:56Results: PMN Migration Across Bladder Epithelia in Response to Various Stimuli
  • 10:59Conclusion

Summary

Automatic Translation

私たちは、尿路病原性大腸菌で膀胱の感染中の急性の炎症反応の重要な要素を模倣したin vitroモデルを開発しました。 transuroepithelial好中球遊走アッセイは、細菌感染や化学誘引物質に反応して、透過性の支持体上で培養、膀胱上皮間でヒト好中球の遊走の定量的評価が可能になります。

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