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使用穆林精密切肺片对肺动脉内下氧肺血管收缩进行视频形态分析
JoVE Journal
Medicine
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JoVE Journal Medicine
Videomorphometric Analysis of Hypoxic Pulmonary Vasoconstriction of Intra-pulmonary Arteries Using Murine Precision Cut Lung Slices
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使用穆林精密切肺片对肺动脉内下氧肺血管收缩进行视频形态分析

DOI:
10.3791/50970-v

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13:32 min

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January 14, 2014

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, , ,
1Institute of Anatomy and Cell Biology,Justus-Liebig-University

Chapters

  • 00:05Title
  • 01:44Isolation of Murine Lungs and Preparation of Precision Cut Lung Slices
  • 06:05Videomorphometric Analysis of Intrapulmonary Arteries of Precision Cut Lung Slices
  • 08:50Analysis of Vasoreactivity and Graphic Presentation
  • 09:30Results: Hypoxia-induced Vasoconstriction of Pre-acinar and Intra-acinar Arteries
  • 12:42Conclusion

Summary

Automatic Translation

Automatic Translation

缺氧肺血管收缩（HPV）是一种重要的生理现象，通过它，在肺气孔缺氧与通气匹配。促成HPV的主要血管部分是血管内动脉。在这里，我们描述了我们的方案，分析直径为20-100微米的murine肺血管的HPV。

Tags

Keywords: Videomorphometric AnalysisHypoxic Pulmonary VasoconstrictionIntra-pulmonary ArteriesMurine Precision Cut Lung SlicesIntra-acinar ArteriesPre-acinar ArteriesVasoreactivityAlveolar HypoxiaVon Euler-Liljestrand MechanismPulmonary PerfusionVentilation Matching

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