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Immunology and Infection
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Automated Separation of C. elegans Variably Colonized by a Bacterial Pathogen
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Immunology and Infection
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JoVE Journal Immunology and Infection
Automated Separation of C. elegans Variably Colonized by a Bacterial Pathogen

Automated Separation of C. elegans Variably Colonized by a Bacterial Pathogen

DOI:
10.3791/51090-v

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11:18 min

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March 21, 2014

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, ,
1Department of Integrative Biology,University of California, Berkeley

Chapters

  • 00:05Title
  • 01:49Infection of Synchronized Young Adult Worms with Pseudomonas aeruginosa
  • 04:44Setting up the Wormsorter
  • 06:19Sorting Colonized vs Noncolonized Worms
  • 08:28Results: Colonization is Not Required for the Transcriptional Response to P. aeruginosa
  • 10:19Conclusion

Summary

Automatic Translation

Automatic Translation

The wormsorter facilitates genetic screens in Caenorhabditis elegans by sorting worms according to expression of fluorescent reporters. Here, we describe a new usage: sorting according to colonization by a GFP-expressing pathogen, and we employ it to examine the poorly understood role of pathogen recognition in initiating immune responses.

Tags

Keywords: C. ElegansCaenorhabditis ElegansBacterial PathogenWormsorterFACSGFPPseudomonas AeruginosaImmune ResponsePathogen ColonizationPathogen-associated Molecular PatternsDamage Detection

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