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Neuroscience
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细胞表面和内蛋白质的现场培养神经细胞的抗体后喂养差异化标记
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons
Please note that all translations are automatically generated.
Click here for the English version.
细胞表面和内蛋白质的现场培养神经细胞的抗体后喂养差异化标记
DOI:
10.3791/51139-v
•
11:56 min
•
February 12, 2014
•
Nissa L. Carrodus*
1
,
Kathleen Sue-Lyn Teng*
1
,
Kathryn M. Munro
,
Matthew J. Kennedy
,
Jenny M. Gunnersen
3
1
Department of Anatomy & Neuroscience
,
The University of Melbourne
,
2
Department of Neurobiology
,
Duke University Medical Center
,
3
Florey Institute of Neuroscience & Mental Health
,
The University of Melbourne
Chapters
00:05
Title
01:28
Preparation of Dissociated Primary Embryonic Neuron Culture
04:56
Antibody Incubation with Live Neurons
10:26
Representative Imaging Results
11:33
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一种方法,以使用特定的多克隆抗体对表位的细胞外活神经元的表面上的标记蛋白。蛋白结合的抗体在细胞表面上,并随后通过胞吞作用内在化可以从蛋白质上残留进行区分,或在培养过程中贩卖到,该表面。
Tags
Differential Labeling
Cell-surface Proteins
Internalized Proteins
Antibody Feeding
Live Cultured Neurons
Transmembrane Receptor
Protein Trafficking
Endocytosis
Fluorescent Secondary Antibody
Subcellular Localization
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