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13:52 min
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March 19, 2014
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Summary
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Kindlins är grundläggande för celladhesion via integriner men studier av dem har hämmats av svårigheterna i att uttrycka dem rekombinant i bakterievärdar. Vi beskriver här metoder för effektiv produktion i baculovirus-infekterade insektsceller.
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Cite this Article
Yates, L. A., Gilbert, R. J. C. Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies. J. Vis. Exp. (85), e51206, doi:10.3791/51206 (2014).
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