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Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells
 

Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells

Article DOI: 10.3791/55756-v 12:09 min April 20th, 2017
April 20th, 2017

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Summary

Nonlytic insect cell expression systems are underutilized for production, cellular trafficking/localization, and recombinant protein functional analysis. Here, we describe methods to generate expression vectors and subsequent transient protein expression in commercially available lepidopteran cell lines. The co-localization of Bemisia tabaci aquaporins with subcellular fluorescent marker proteins is also presented.

Tags

Keywords: Insect Cells Recombinant Protein Expression Fluorescent Tagging Protein Function Protein Localization Cell Culture Transfection SF9 Cells Tni Cells Serum-free Medium TNM-FH Medium Cell Passage
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