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Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells
 

Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells

Article doi: 10.3791/55756
April 20th, 2017 Usage Statistics

Summary April 20th, 2017

Nonlytic insect cell expression systems are underutilized for production, cellular trafficking/localization, and recombinant protein functional analysis. Here, we describe methods to generate expression vectors and subsequent transient protein expression in commercially available lepidopteran cell lines. The co-localization of Bemisia tabaci aquaporins with subcellular fluorescent marker proteins is also presented.

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