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高效的生产和纯化重组小鼠Kindlin-3从昆虫细胞生物物理研究
JoVE Journal
Biology
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JoVE Journal
Biology
Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies
Please note that all translations are automatically generated.
Click here for the English version.
高效的生产和纯化重组小鼠Kindlin-3从昆虫细胞生物物理研究
DOI:
10.3791/51206-v
•
13:52 min
•
March 19, 2014
•
Luke A. Yates
,
Robert J. C. Gilbert
1
Division of Structural Biology, Wellcome Trust Centre for Human Genetics
,
University of Oxford
Chapters
00:05
Title
01:13
Generation of Recombinant Baculovirus
04:57
Amplification of Recombinant Baculovirus
06:23
Expression of Kindlin-3 in Baculovirus-infected Sf9
07:39
Purification of Recombinant Kindlin-3
09:33
Results: Milligram Quantitites of Recombinant Murine Kindlin-3 Produced from Insect Cells
13:21
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
Kindlins是基本的细胞粘附通过整合,但他们的研究已经阻碍了在细菌宿主中重组表达他们所遇到的困难。我们在这里描述它们高效的生产在杆状病毒感染的昆虫细胞的方法。
Tags
Recombinant Murine Kindlin-3
Insect Cells
Baculovirus
Sf9 Cells
Protein Purification
Biophysical Studies
Integrins
Cell Signaling
Platelet Aggregation
Leukocyte Rolling
Osteoclast Formation
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