Journal
/
Environment
/
使用吉布森大会和基因枪烟草中瞬时基因表达
JoVE Journal
Environment
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Environment
Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun
Please note that all translations are automatically generated. Click here for the English version.

使用吉布森大会和基因枪烟草中瞬时基因表达

DOI:
10.3791/51234-v

•

12:02 min

•

April 18, 2014

•

, , ,
1Synthetic Biology Platform, Wyss Institute for Biologically Inspired Engineering,Harvard University, 2Department of Systems Biology,Harvard Medical School, 3Department of Biotechnology,Delft University of Technology

Chapters

  • 00:05Title
  • 01:11Design of Sequences for Transient Expression
  • 02:06Gibson Assembly of DNA Parts
  • 06:16Biolistic Transfection of Tobacco with the Gene Gun
  • 08:50Representative Images of Biolistically Transfected Tobacco Leaf Cells
  • 11:18Conclusion

Summary

Automatic Translation

Automatic Translation

这个工作描述了一种新颖的方法，用于选择性地靶向亚细胞器在植物中，使用BioRad公司基因枪进行测定。

Tags

Alternative SplicingChloroplastCytosolGibson AssemblyGFPGene GunMetabolic EngineeringMulti-organelle TargetingPeroxisomeSynthetic BiologyTobaccoTransient Gene Expression

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

在使用高通量微孔板测定水，土壤，沉积物和微生物测定胞外酶活性的

成绩单及代谢物谱烟草树和杨树的评价作为原料的生物基工业

跟踪微生物污染在零售环境中使用荧光粉 - 零售熟食店环境范例

重组纤维素酶Cel5A从表达<em>里氏木霉</em>在烟草

食品筛查为1类整合和基因盒

黄曲霉毒素在花生RNAi介导的控制：方法分析霉菌毒素的产生和转基因表达的花生/<em>黑</em>病害系统

藤壶Balanus improvisus作为海洋模型的培养和基因表达

农杆菌和根瘤菌--马铃薯介导的马铃薯转化与葡萄染色诱导素基因的启动子活性

分离叶测定,以简化马铃薯中基因表达研究,在咀嚼昆虫曼杜卡性塔感染

在纯落叶支架中使用三种截然不同的方法估算叶面积指数

Read Article