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表达,分离,纯化可溶性和不溶性蛋白质生物素对神经组织再生
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration
Please note that all translations are automatically generated.
Click here for the English version.
表达,分离,纯化可溶性和不溶性蛋白质生物素对神经组织再生
DOI:
10.3791/51295-v
•
12:03 min
•
January 22, 2014
•
Aleesha M. McCormick
,
Natalie A. Jarmusik
,
Elizabeth J. Endrizzi
,
Nic D. Leipzig
1
Department of Chemical and Biomolecular Engineering
,
University of Akron
Chapters
00:05
Title
02:55
Bacterial Cell Culture
04:25
Isolation and Purification of Recombinant Protein
05:42
Ni-NTA Affinity Chromatography
08:31
Results: Purification of Native and Nonnative Proteins for Biotinylation
10:32
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
开发biotinylatable融合蛋白具有在研究各个领域的许多潜在的应用。重组蛋白质工程是直线前进的过程,是符合成本效益的,提供定制设计的蛋白质的产量高。
Tags
Recombinant Protein Engineering
Escherichia Coli
Biotin Tag
Streptavidin
Green Fluorescent Protein
Unnatural Amino Acids
Metal Ion Chelators
Post-translational Modifications
Fusion Proteins
Biotinylatable Protein
Nerve Growth Factor
Semaphorin3A
Biomaterials
Regenerative Medicine
T7 Lac Inducible Vector
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