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Endothelial Cell Tube Dannelse Assay for<em> In Vitro</em> Study of Angiogenese
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JoVE Journal Biology
Endothelial Cell Tube Formation Assay for the In Vitro Study of Angiogenesis

Endothelial Cell Tube Dannelse Assay for<em> In Vitro</em> Study of Angiogenese

DOI:
10.3791/51312-v

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08:12 min

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September 01, 2014

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, , , , , , , , , ,
1Department of Biology,American University, 2Laboratory of Cancer Biology and Genetics,National Cancer Institute, NIH

Chapters

  • 00:05Title
  • 01:29Growing and Starving Endothelial Cells
  • 02:07Preparing Reagents
  • 02:37Preparing Endothelial Cells
  • 03:35Tube Formation Assay
  • 05:59Results: Endothelial Tube Formation
  • 07:46Conclusion

Summary

Automatic Translation

Automatic Translation

Røret dannelse analysen er en rask, kvantifiserbare metode for å måle in vitro angiogenese. Endotelceller er kombinert med kondisjonert media og sådd ut på basalmembran ekstrakt. Tube dannelsen oppstår i løpet av timer og nydannede tubuli lett kvantifisert.

Tags

Endothelial CellTube FormationAngiogenesisIn VitroBasement Membrane MatrixConditioned MediaCapillary-like StructuresBranch SitesLoopsMeshesTube LengthQuantification

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