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木质素下调<em>玉米</em>通过dsRNAi和Klason木质素分析

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis

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木质素下调<em>玉米</em>通过dsRNAi和Klason木质素分析

DOI:

10.3791/51340-v

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14:43 min

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July 23, 2014

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Sang-Hyuck Park, Rebecca Garlock Ong, Chuansheng Mei, Mariam Sticklen

¹The School of Plant Sciences,University of Arizona, ²Department of Chemical Engineering and Materials Science, DOE Great Lakes Bioenergy Research Center,Michigan State University, ³The Institute for Sustainable and Renewable Resources,The Institute for Advanced Learning and Research, ⁴Department of Plant, Soil and Microbial Sciences,Michigan State University

Chapters

00:05Title
02:37Maize Genetic Transformation
06:42Maize Regeneration
08:02Klason Lignin Measurement
11:40Results: dsRNAi Downregulation of Lignin
13:32Conclusion

Summary

Automatic Translation

双链RNA干扰（dsRNAi）技术以下调玉米肉桂酰辅酶A还原酶（ZmCCR1）基因为低植物木质素含量。木质素下调从细胞壁是由微观分析和可视化的Klason法进行定量。在半纤维素和结晶纤维素组成的变化进行了分析。

Tags

Lignin Zea Mays DsRNAi Klason Lignin Analysis Cell Wall Cellulose Hemicellulose Cinnamoyl-CoA Reductase ZmCCR1 Bioenergy Pretreatment Bioethanol Production

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