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Lignin nedregulering av<em> Zea mays</em> Via dsRNAi og Klason Lignin Analysis
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1The School of Plant Sciences,University of Arizona, 2Department of Chemical Engineering and Materials Science, DOE Great Lakes Bioenergy Research Center,Michigan State University, 3The Institute for Sustainable and Renewable Resources,The Institute for Advanced Learning and Research, 4Department of Plant, Soil and Microbial Sciences,Michigan State University
Chapters
- 00:05Title
- 02:37Maize Genetic Transformation
- 06:42Maize Regeneration
- 08:02Klason Lignin Measurement
- 11:40Results: dsRNAi Downregulation of Lignin
- 13:32Conclusion
En dobbelttrådet RNA interferens (dsRNAi) teknikken er ansatt for å ned-regulere mais cinnamoylforbindelser koenzym A-reduktase (ZmCCR1) genet til lavere plante lignin innhold. Lignin nedregulering av celleveggen blir visualisert ved mikroskopiske analyser og kvantifiseres ved Klason metoden. Sammensetningsendringer hemicellulose og krystallinsk cellulose blir analysert.
