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Lignine Neerregeling van<em> Zea mays</em> Via dsRNAi en Klason Lignine Analyse
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1The School of Plant Sciences,University of Arizona, 2Department of Chemical Engineering and Materials Science, DOE Great Lakes Bioenergy Research Center,Michigan State University, 3The Institute for Sustainable and Renewable Resources,The Institute for Advanced Learning and Research, 4Department of Plant, Soil and Microbial Sciences,Michigan State University
Chapters
- 00:05Title
- 02:37Maize Genetic Transformation
- 06:42Maize Regeneration
- 08:02Klason Lignin Measurement
- 11:40Results: dsRNAi Downregulation of Lignin
- 13:32Conclusion
Een dubbelstrengs RNA interferentie (dsRNAi) techniek wordt toegepast om down-reguleren van de maïs cinnamoyl co-enzym A-reductase (ZmCCR1) gen tot lagere planten ligninegehalte. Lignine down-regulatie van de celwand wordt gevisualiseerd door microscopische analyses en gekwantificeerd door de Klason methode. Compositionele veranderingen in hemicellulose en cellulose geanalyseerd.
