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Isolering og dyrkning af Voksen Mouse kardiomyocytter for cellesignalering og<em> In vitro</em> Hjertehypertrofi
JoVE Journal
Biology
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JoVE Journal Biology
Isolation and Culture of Adult Mouse Cardiomyocytes for Cell Signaling and in vitro Cardiac Hypertrophy
DOI:

06:51 min

May 21, 2014

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Chapters

  • 00:05Title
  • 01:12Mouse Heart Removal and Cannulation
  • 02:54Heart Perfusion and Digestion
  • 03:25Cells Dissociation and Calcium Reintroduction
  • 05:10Cell Culture
  • 06:00Results: Successful Isolation Quantification
  • 06:29Conclusion

Summary

Automatic Translation

Vi beskriver en pålidelig metode til isolering af voksne mus cardiomyocytter. Denne protokol giver et ensartet resultat til dyrkning af funktionelle voksne kardiomyocytter fra en række af genetisk modificerede mus.

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