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細胞シグナリングのための成体マウスの心筋細胞の単離と文化<em> in vitroで</em>心肥大
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Isolation and Culture of Adult Mouse Cardiomyocytes for Cell Signaling and
in vitro
Cardiac Hypertrophy
Please note that all translations are automatically generated.
Click here for the English version.
細胞シグナリングのための成体マウスの心筋細胞の単離と文化<em> in vitroで</em>心肥大
DOI:
10.3791/51357-v
•
06:51 min
•
May 21, 2014
•
Daxiang Li
,
Jian Wu
,
Yan Bai
,
Xiaochen Zhao
,
Lijun Liu
1
Department of Biochemistry and Cancer Biology
,
University of Toledo College of Medicine and Life Sciences
,
2
Department of Physiology and Pharmacology
,
University of Toledo College of Medicine and Life Sciences
Chapters
00:05
Title
01:12
Mouse Heart Removal and Cannulation
02:54
Heart Perfusion and Digestion
03:25
Cells Dissociation and Calcium Reintroduction
05:10
Cell Culture
06:00
Results: Successful Isolation Quantification
06:29
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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italiano (Italian)
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português (Portuguese)
русский (Russian)
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Automatic Translation
我々は、成体マウス心筋細胞の単離のための信頼できる方法を記載している。このプロトコルは、遺伝子改変マウスの様々から機能大人の心筋細胞の培養のための一貫性のある結果が得られます。
Tags
Adult Mouse Cardiomyocytes
Cell Signaling
Cardiac Hypertrophy
Genetically Modified Mice
Cardiomyocyte Isolation
Langendorff Perfusion
Type II Collagenase
Cardiovascular Research
Drug Development
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