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使用α-银环蛇毒素结合位点标记来研究GABA A受体膜定位和贩运
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Using an α-Bungarotoxin Binding Site Tag to Study GABA A Receptor Membrane Localization and Trafficking
Please note that all translations are automatically generated.
Click here for the English version.
使用α-银环蛇毒素结合位点标记来研究GABA A受体膜定位和贩运
DOI:
10.3791/51365-v
•
11:57 min
•
March 28, 2014
•
Megan L. Brady
,
Charles E. Moon
,
Tija C. Jacob
1
Pharmacology & Chemical Biology Department
,
University of Pittsburgh School of Medicine
Chapters
00:05
Title
02:08
Preparation of Hippocampal Neuronal Cultures
03:13
Endocytosis Assay
07:23
Image Acquisition
08:26
Results: Visualizing Surface GABAAR Localization and Endocytosis with Fluorescent α-Bungarotoxin
11:03
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们展示了使用荧光染料的Alexa耦合到α-银环蛇毒素测定GABA A受体表面的定位和内吞作用的海马神经元。通过采用轴承结构结合α-银环蛇毒素,可以实现质膜蛋白的内吞贩运分析短外标签。
Tags
α-Bungarotoxin
GABA A Receptor
Membrane Localization
Trafficking
BBS Tag
PH-sensitive GFP
Receptor Endocytosis
Receptor Surface Mobility
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