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患者とヒト内皮細胞に由来する前立腺循環腫瘍細胞との間のE-セレクチン介在性の相互作用を観察する
in vitro
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JoVE Journal
Medicine
In vitro
Method to Observe E-selectin-mediated Interactions Between Prostate Circulating Tumor Cells Derived From Patients and Human Endothelial Cells
Please note that all translations are automatically generated.
Click here for the English version.
患者とヒト内皮細胞に由来する前立腺循環腫瘍細胞との間のE-セレクチン介在性の相互作用を観察する
in vitro
法
での
DOI:
10.3791/51468-v
•
11:56 min
•
May 15, 2014
•
Gunjan Gakhar
,
Neil H. Bander
,
David M. Nanus
1
Department of Medicine
,
Weill Cornell Medical College
,
2
Department of Urology
,
Weill Cornell Medical College
Chapters
00:05
Title
01:50
Culturing HUVECs on Microslides for Observing Circulating Tumor Cell (CTC)-endothelial Interactions
04:04
Preparation of Flow Chamber Assembly for Overnight HUVEC Culture on a Microslide
05:48
Preparation of Anti-PSMA Labeled Prostate Cancer Cells
07:05
Preparation of Microscope, Syringe Pump and Flow Chamber Assembly
09:11
Results: Analysis of Interactions Between Labeled MDA Prostate Cancer Cells and Endothelial Cells
11:11
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我々の報告は、生理的流れの条件下で、前立腺癌においてCTC / ECの相互作用を可視化し、分析するためのユニークな方法を記載する。
Tags
In Vitro
E-selectin
Prostate Circulating Tumor Cells
Human Endothelial Cells
Metastasis
Flow Chamber
Circulating Tumor Cells
Prostate Cancer Cells
Cell Interactions
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