Journal
/
Bioengineering
/
In vitro-cellekultur Model for Toxic Inhaleret Chemical Testing
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
In vitro Cell Culture Model for Toxic Inhaled Chemical Testing

In vitro-cellekultur Model for Toxic Inhaleret Chemical Testing

DOI:
10.3791/51539-v

•

05:44 min

•

May 08, 2014

•

, , , , , ,
1Pediatric Airway Research Center, Department of Pediatrics,University of Colorado, 2Department of Chemical and Biological Engineering,Colorado School of Mines

Chapters

  • 00:05Title
  • 01:12Chlorine Exposure and Transepithelial Electrical Resistance (TER) Measurement
  • 03:35Results: Representative Effects of Chlorine Exposure on Rat and Human Cells
  • 05:20Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol er udviklet til at påvise eksponering metode cellekulturer til inhaleret giftige kemikalier. Eksponering af differentieret luft-væske grænsefladen (ALI) kulturer af luftvejs epitelceller giver en unik model af luftvejene eksponering for giftige gasser såsom klor. I dette manuskript beskriver vi effekten af klor eksponering på luft-væske grænsefladen kulturer af epitelceller og nedsænket kultur cardiomyocytes. In vitro eksponering systemer giver vigtige mekanistiske undersøgelser for at evaluere veje, der kunne så bruges til at udvikle nye terapeutiske midler.

Tags

In Vitro Cell CultureToxic Inhaled ChemicalsAirway EpitheliumHeart Muscle CellsChlorine ExposureMembrane IntegrityCaspase ReleaseCell Death

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

A Method for Ovarian Follicle Encapsulation and Culture in a Proteolytically Degradable 3 Dimensional System

Shape Memory Polymers for Active Cell Culture

Autologous Endothelial Progenitor Cell-Seeding Technology and Biocompatibility Testing For Cardiovascular Devices in Large Animal Model

Roterende Cell Culture Systems for Human Cell Culture: Human trofoblasten celler som en model

Øjenirritation Test (EIT) for Hazard Identifikation af Eye Irriterer Kemikalier hjælp rekonstrueret human Cornea-lignende Epithelial (RHCE) Tissue Model

Light-medieret dannelse og mønsterdannelse af hydrogeler til celledyrkningsapplikationer

en<em> In vitro</em> Orgel Kultur Model af muse diskusprolaps

Metodologi for biomimetiske kemiske Neuromodulationsbehandling af rotte nethinder med neurotransmitteren glutamat In Vitro

En menneskelig 3D ekstracellulær matrix-Adipocyte kultur model for at studere matrix-celle metaboliske Crosstalk

Read Article