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为获取原发性卵巢癌细胞固体样品的方法
JoVE Journal
Medicine
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JoVE Journal
Medicine
Method for Obtaining Primary Ovarian Cancer Cells From Solid Specimens
Please note that all translations are automatically generated.
Click here for the English version.
为获取原发性卵巢癌细胞固体样品的方法
DOI:
10.3791/51581-v
•
05:58 min
•
February 04, 2014
•
Lee J. Pribyl
,
Kathleen A. Coughlin
,
Thanasak Sueblinvong
,
Kristin Shields
,
Yoshie Iizuka
3
,
Levi S. Downs
3
,
Rahel G. Ghebre
3
,
Martina Bazzaro
3
1
Department of Obstetrics, Gynecology, and Women’s Heath
,
University of Minnesota
,
2
Department of Obstetrics and Gynecology
,
Maricopa Medical Center and St Josephs Hospital and Medical Center
,
3
Masonic Cancer Center
,
University of Minnesota
Chapters
00:05
Title
01:34
Reagent Setup
02:00
Tissue Processing
04:45
Results: Cell Culture Profile
05:37
Conclusion
Summary
Automatic Translation
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Automatic Translation
这项研究描述了隔离原发性卵巢癌细胞从固体临床标本和表征了详细的方法。卵巢癌的临床标本进行酶消化，以获取可行的，成纤维细胞无上皮性卵巢癌（EOC）细胞非常适合用于下游应用。
Tags
Primary Ovarian Cancer Cells
Solid Specimens
Cell Isolation
Enzymatic Digestion
Cell Culture
Drug Screening
Ovarian Cancer Cell Lines
Heterogeneity
Genetic Alterations
In Vitro Passaging
Clinical Specimens
Metastatic Ovarian Cancer
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