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JoVE Journal Biology
Aislamiento de Neonatal extrahepática cholangiocytes
 
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Aislamiento de Neonatal extrahepática cholangiocytes

Article doi: 10.3791/51621
June 5th, 2014 •
1, 2
1Division of Gastroenterology, Hepatology, and Nutrition, The Children's Hospital of Philadelphia, 2Department of Medicine, The Perelman School of Medicine at the University of Pennsylvania

Summary
Automatic Translation
June 5th, 2014

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Una técnica para aislar cholangiocytes de los conductos biliares extrahepáticos de ratones recién nacidos se describe. Los conductos están meticulosamente disecados, y luego las células se aíslan por consecuencia en geles de colágeno gruesas. Este método proporciona una herramienta útil para el estudio del desarrollo de vías biliares extrahepáticas y patología.

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