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Isolering af Neonatal Ekstrahepatisk Cholangiocytes
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JoVE Journal Biology
Isolation of Neonatal Extrahepatic Cholangiocytes

Isolering af Neonatal Ekstrahepatisk Cholangiocytes

DOI:
10.3791/51621-v

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07:54 min

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June 05, 2014

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,
1Division of Gastroenterology, Hepatology, and Nutrition,The Children’s Hospital of Philadelphia, 2Department of Medicine,The Perelman School of Medicine at the University of Pennsylvania

Chapters

  • 00:05Title
  • 01:12Bile Duct Isolation
  • 02:59Embedding Tissues into Thick Collagen Gels
  • 04:19Isolating Primary Cholangiocytes
  • 07:05Characterizing Cultured Cholangiocytes
  • 07:24Conclusion

Summary

Automatic Translation

Automatic Translation

En teknik til at isolere cholangiocytes fra ekstrahepatiske galdegange af neonatale mus er beskrevet. Kanalerne er omhyggeligt dissekeret, og cellerne isoleres ved udvækst i tykke kollagengeler. Denne metode giver et nyttigt værktøj til at studere ekstrahepatisk galdegang udvikling og patologi.

Tags

Neonatal Extrahepatic CholangiocytesBile DuctsIntrahepaticExtrahepaticCell IsolationCollagenCell CultureTrypsinizationCell PurificationBiliary Atresia

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