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Methods to Characterize Spontaneous and Startle-induced Locomotion in a Rotenone-induced Parkinson’s Disease Model of Drosophila
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Neuroscience
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JoVE Journal Neuroscience
Methods to Characterize Spontaneous and Startle-induced Locomotion in a Rotenone-induced Parkinson’s Disease Model of Drosophila

Methods to Characterize Spontaneous and Startle-induced Locomotion in a Rotenone-induced Parkinson’s Disease Model of Drosophila

DOI:
10.3791/51625-v

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07:58 min

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August 17, 2014

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, ,
1Department of Biology,Colby College

Chapters

  • 00:05Title
  • 01:12Startle-induced Locomotion Assay and Setup
  • 03:11Startle-induced Locomotion Experimentation and Data Analysis
  • 04:04Spontaneous Locomotion Assay Setup
  • 05:19Spontaneous Locomotion Data Collection and Analysis
  • 06:36Results: Rotenone-induced Response Deficiency
  • 07:35Conclusion

Summary

Automatic Translation

Automatic Translation

Parkinson’s disease is a neurodegenerative disorder that results from the degeneration of dopaminergic neurons in the central nervous system, causing locomotion defects. Rotenone models Parkinson’s disease in Drosophila. This paper outlines two assays that characterize both spontaneous and startle-induced locomotion deficiencies caused by rotenone.

Tags

Keywords: Parkinson’s DiseaseDrosophilaRotenoneLocomotionStartle ResponseSpontaneous MovementMotor DeficienciesNeurodegenerative DisorderDopaminergic NeuronsOxidative DamageMitochondriaNegative Geotactic ResponseHigh-throughput Assays

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