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单通道细胞贴附膜片钳记录
JoVE Journal
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One-channel Cell-attached Patch-clamp Recording
Please note that all translations are automatically generated.
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单通道细胞贴附膜片钳记录
DOI:
10.3791/51629-v
•
13:07 min
•
June 09, 2014
•
Bruce A. Maki
,
Kirstie A. Cummings
,
Meaghan A. Paganelli
,
Swetha E. Murthy
,
Gabriela K. Popescu
1
Graduate Program in Neuroscience, SMBS
,
University at Buffalo, SUNY
,
2
Department of Biochemistry, SMBS
,
University at Buffalo, SUNY
,
3
Molecular and Cellular Neuroscience Department
,
The Scripps Research Institute
,
4
Department of Biochemistry and Graduate Program in Neuroscience, SMBS
,
University at Buffalo, SUNY
Chapters
00:05
Title
01:53
Cell Culture and Protein Expression
03:06
Cell-Attached Patch-Clamp Recording
07:21
Data Preprocessing and Idealization
11:22
Results: One-Channel Recordings
12:25
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里描述的是获得当前记录的很长一段距离与细胞贴附膜片钳技术一种离子通道的过程。这种方法允许观察,实时的背后的生物信号开闭通道构象的图案。这些数据的通知关于在不受干扰的生物膜通道属性。
Tags
Ion Channel
Patch-clamp
Single-channel Recording
NMDA Receptor
PIEZO1
HEK293 Cells
Cortical Neurons
Conductance
Open-closed Conformations
Activation Mechanism
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