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1Graduate Program in Neuroscience, SMBS,University at Buffalo, SUNY, 2Department of Biochemistry, SMBS,University at Buffalo, SUNY, 3Molecular and Cellular Neuroscience Department,The Scripps Research Institute, 4Department of Biochemistry and Graduate Program in Neuroscience, SMBS,University at Buffalo, SUNY
Chapters
- 00:05Title
- 01:53Cell Culture and Protein Expression
- 03:06Cell-Attached Patch-Clamp Recording
- 07:21Data Preprocessing and Idealization
- 11:22Results: One-Channel Recordings
- 12:25Conclusion
Beskrevet her er en procedure for at opnå lange strækninger af aktuelle optagelse fra en ion-kanal med celle-attached patch-clamp teknik. Denne metode giver mulighed for at observere, i realtid, mønsteret for open-close kanal konformationer, der ligger til grund for biologisk signal. Disse data informere om kanal ejendomme i uforstyrrede biologiske membraner.
