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A New Clarification Method to Visualize Biliary Degeneration During Liver Metamorphosis in Sea Lamprey (Petromyzon marinus)
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JoVE Journal Biology
A New Clarification Method to Visualize Biliary Degeneration During Liver Metamorphosis in Sea Lamprey (Petromyzon marinus)

A New Clarification Method to Visualize Biliary Degeneration During Liver Metamorphosis in Sea Lamprey (Petromyzon marinus)

DOI:
10.3791/51648-v

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07:03 min

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June 06, 2014

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, , , , , , ,
1Department of Fisheries & Wildlife,Michigan State University, 2Hammond Bay Biological Station, Great Lakes Science Center,U.S. Geological Survey

Chapters

  • 00:05Title
  • 01:43Solution and Tissue Preparation
  • 04:59Results: Biliary Degeneration During Metamorphosis in Clarified Sea Lamprey
  • 06:27Conclusion

Summary

Automatic Translation

Automatic Translation

Sea lamprey lose the gall bladder and bile ducts during metamorphosis, a process similar to human biliary atresia. A new fixation and clarification method (CLARITY) was modified to visualize the entire biliary tree using laser scanning confocal microscopy. This method provides a powerful tool to study biliary degeneration.

Tags

Biliary DegenerationLiver MetamorphosisSea LampreyPetromyzon MarinusCLARITYConfocal MicroscopyBiliary Atresia

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