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Vurdering af kræft i æggestokkene Sfæroide Attachment og invasion af mesotelceller i Real Time
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
Assessment of Ovarian Cancer Spheroid Attachment and Invasion of Mesothelial Cells in Real Time
DOI:

14:25 min

May 20, 2014

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Chapters

  • 00:05Title
  • 02:05Optional Fluorescent Cell Labeling Method
  • 03:24Generation of Spheroids in Methylcellulose
  • 04:28Real Time Cell Analyzer (RTCA) Cell Invasion Assay
  • 05:46Programming the RTCA Instrument
  • 07:25Generation of LP9 Monolayer and Measurement of Spheroid Invasion
  • 09:49Results: Real Time Assessment of Ovarian Cancer Spheroid Invasion
  • 13:42Conclusion

Summary

Automatic Translation

Æggestokkene cancercelleinvasion i mesothelial foring af bughinden er en dynamisk proces over tid. Ved hjælp af en realtid analysator, kan den invasive kapacitet kræft i æggestokkene celler i en kugleformet-mesothelial celle co-kultur model kvantificeres over længere tidsperioder, der giver indsigt i faktorer, der regulerer metastatisk proces.

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