gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair

Sandy Chevrier; Romain Boidot

doi:10.3791/51902

Journal

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Biology

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基因组DNA富集转座为基础的技术的整个序列的农工商分析 BRCA1基因 BRCA2和9个基因参与DNA损伤修复

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Biology

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JoVE Journal Biology

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair

Please note that all translations are automatically generated. Click here for the English version.

基因组DNA富集转座为基础的技术的整个序列的农工商分析 BRCA1基因 BRCA2和9个基因参与DNA损伤修复

DOI:

10.3791/51902-v

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08:15 min

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October 06, 2014

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Sandy Chevrier, Romain Boidot

¹Department of Biology and Pathology of Tumors, Unit of Molecular Biology, Platform of Immunomonitoring and Genetics,Centre Georges-François Leclerc

Chapters

00:05Title
01:14gDNA Enrichment: Day 1, Morning
03:11gDNA Enrichment: Day 1, Afternoon
03:50gDNA Enrichment: Day 2, Morning
06:16gDNA Enrichment: Day 2, Afternoon and Day 3
07:19Results: gDNA Enrichment by Transposase-based Technology
07:55Conclusion

Summary

Automatic Translation

gDNA enrichment for NGS sequencing is an easy and powerful tool for the study of constitutional mutations. In this article, we present the procedure to analyse simply the complete sequence of 11 genes involved in DNA damage repair.