JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Developmental Biology
/
荧光蛋白的表达<em>披文昌鱼</em>通过基因注射到未受精的卵母细胞
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Developmental Biology
Expression of Fluorescent Proteins in
Branchiostoma lanceolatum
by mRNA Injection into Unfertilized Oocytes
Please note that all translations are automatically generated.
Click here for the English version.
荧光蛋白的表达<em>披文昌鱼</em>通过基因注射到未受精的卵母细胞
DOI:
10.3791/52042-v
•
09:31 min
•
January 12, 2015
•
Estelle Hirsinger
,
João Emanuel Carvalho
,
Christine Chevalier
3
,
Georges Lutfalla
,
Jean-François Nicolas
,
Nadine Peyriéras
,
Michael Schubert
1
Département de Biologie du Développement et Cellules Souches
,
Institut Pasteur
,
2
Laboratoire de Biologie du Développement de Villefranche-sur-Mer (UMR7009 CNRS/UPMC Univ Paris 06)
,
Sorbonne Universités
,
3
Equipe Epigenetic Control of Normal and Pathological Hematopoiesis
,
Centre de Recherche en Cancérologie de Marseille
,
4
Unité de Dynamique des Interactions Membranaires Normales et Pathologiques
,
CNRS UMR5235/DAA/cc107/Université Montpellier II
,
5
Plateforme BioEmergences IBiSA FBI
,
CNRS-NED, Institut de Neurobiologie Alfred Fessard
Chapters
00:05
Title
01:21
Poly-lysine Coated Dishes
02:04
Agarose Coated Dishes
02:38
Injection Mix and Loading Injection Needles
03:40
Sample Collection
04:38
Oocyte Injection
06:39
Fertilization, Selection, and Embryo Culture
07:55
Results: Expression of Fluorescent Proteins After mRNA Injection
08:56
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
我们在这里报告的强大和高效表达荧光蛋白的mRNA注射后进入
披文昌鱼
的未受精的卵母细胞。显微注射技术在此基础脊索动物的发展铺平道路,在这个新兴的模型系统深远的技术创新,包括
在体内
成像和基因特异的操作。
Tags
Fluorescent Proteins
MRNA Injection
Unfertilized Oocytes
Branchiostoma Lanceolatum
Amphioxus
MCherry
EGFP
Codon Usage
Kozak Sequence
Microinjection
Live Imaging
Cell Behavior
Embryonic Development
Evolution
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
操作和<em>在体外</em中>成熟<em>非洲爪蟾</em>卵母细胞,其次是胞浆内单精子注射,研究胚胎发育
基因转移到鸡听觉器官通过<em>在卵</em>微电
通过注射心脏毒素急性骨骼肌再生的诱导
免疫荧光标记的微管和中心蛋白在
体内
肠道组织和 3D
体外
肠道 Organoids
使用mRNA电穿孔快速有效地表达Avian胚胎中的多种蛋白质
通过转录因子的强制表达,人类纤维细胞的造血再编程
植物组织细胞壁中阿拉比诺格拉坦蛋白和皮克廷的荧光免疫化
未成熟费林卵母细胞的最小体积玻璃化
斑马鱼卵巢卵巢中卵泡细胞和卵母细胞的分离
Murine Neural Plate Targeting by In Utero Nano-Injection (NEPTUNE) at Embryonic Day 7.5
Read Article