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先天免疫和癌前细胞间的相互作用使用可诱导的Gal4 / UAS表达系统在斑马鱼幼体皮肤实时成像

JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology

Live Imaging of Innate Immune and Preneoplastic Cell Interactions Using an Inducible Gal4/UAS Expression System in Larval Zebrafish Skin

Please note that all translations are automatically generated. Click here for the English version.

先天免疫和癌前细胞间的相互作用使用可诱导的Gal4 / UAS表达系统在斑马鱼幼体皮肤实时成像

DOI:

10.3791/52107-v

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08:52 min

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February 03, 2015

•

Thomas Ramezani, Derek W. Laux, Isabel R. Bravo, Masazumi Tada, Yi Feng

¹MRC Centre for Inflammation Research, Queen’s Medical Research Institute,The University of Edinburgh, ²Department of Cell & Developmental Biology,University College London

Chapters

00:05Title
01:46Microinjection of Plasmid DNA
04:16Conditional Skin Cell Transformation by 4-Hydroxytamoxifen (4-OHT)
05:34Mounting and Live Imaging of Zebrafish Larvae
07:06Results: Live Imaging of Innate Immune and Preneoplastic Cell Interactions in Larval Zebrafish Skin
08:01Conclusion

Summary

Automatic Translation

研究癌前细胞发展和先天免疫细胞相互作用的最早事件是举足轻重的理解和治疗癌症。在这里，我们描述了一个方法，有条件地诱导上皮细胞转化和先天免疫细胞相互作用与HRAS ^G12V表达的皮肤细胞在斑马鱼幼虫随后的实时成像。

Tags

Keywords: Live Imaging Innate Immune Cell Preneoplastic Cell Zebrafish Skin 4-Hydroxytamoxifen (4-OHT)KalTA4-ERT2/UAS Expression System HRASG12V Tumorigenesis Ectopic Gene Expression

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