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애벌레 Zebrafish의 피부에 유도 GAL4 / UAS 식 시스템을 사용하여 선천성 면역과 종양 전 세포 상호 작용의 라이브 영상
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Chapters
- 00:05Title
- 01:46Microinjection of Plasmid DNA
- 04:16Conditional Skin Cell Transformation by 4-Hydroxytamoxifen (4-OHT)
- 05:34Mounting and Live Imaging of Zebrafish Larvae
- 07:06Results: Live Imaging of Innate Immune and Preneoplastic Cell Interactions in Larval Zebrafish Skin
- 08:01Conclusion
Studying the earliest events of preneoplastic cell progression and innate immune cell interaction is pivotal to understand and treat cancer. Here we describe a method to conditionally induce epithelial cell transformations and the subsequent live imaging of innate immune cell interaction with HRASG12V expressing skin cells in zebrafish larvae.
