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Developmental Biology
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测量生活中的斑马鱼胚胎蛋白质稳定性使用荧光衰减光转化后(FDAP)
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Developmental Biology
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JoVE Journal
Developmental Biology
Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP)
Please note that all translations are automatically generated.
Click here for the English version.
测量生活中的斑马鱼胚胎蛋白质稳定性使用荧光衰减光转化后(FDAP)
DOI:
10.3791/52266-v
•
09:45 min
•
January 28, 2015
•
Katherine W. Rogers
,
Alexander Bläßle
,
Alexander F. Schier
,
Patrick Müller
1
Department of Molecular and Cellular Biology
,
Harvard University
,
2
Systems Biology of Development Group
,
Friedrich Miescher Laboratory of the Max Planck Society
Chapters
00:05
Title
01:27
Generating a Photoconvertible Fusion Construct and Injecting Dechorionated Zebrafish Embryos
03:22
Mounting Zebrafish Embryos for Photoconversion and Confocal Imaging
04:37
Photoconverting and Measuring the Decrease of the Photoconverted Signal
06:47
Analyzing the Data Using PyFDAP
08:18
Results: Half-life of Squint-Dendra2 as Determined by Fluorescence Decay After Photoconversion during Embryogenesis
09:19
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在细胞和组织中的蛋白水平通常紧紧蛋白质生产和清除的平衡调节。使用荧光衰减光转化(FDAP)后,蛋白质的间隙动力学可以通过实验
在体内
测量。
Tags
Protein Stability
Fluorescence Decay After Photoconversion
FDAP
Protein Clearance Kinetics
Photoconvertible Fluorescent Protein
Zebrafish Embryos
Compartmental Analysis
Intra- And Extracellular Half-lives
Secreted Signaling Proteins
In Vivo Protein Dynamics
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