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测量生活中的斑马鱼胚胎蛋白质稳定性使用荧光衰减光转化后(FDAP)
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP)
DOI:

09:45 min

January 28, 2015

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Chapters

  • 00:05Title
  • 01:27Generating a Photoconvertible Fusion Construct and Injecting Dechorionated Zebrafish Embryos
  • 03:22Mounting Zebrafish Embryos for Photoconversion and Confocal Imaging
  • 04:37Photoconverting and Measuring the Decrease of the Photoconverted Signal
  • 06:47Analyzing the Data Using PyFDAP
  • 08:18Results: Half-life of Squint-Dendra2 as Determined by Fluorescence Decay After Photoconversion during Embryogenesis
  • 09:19Conclusion

Summary

Automatic Translation

在细胞和组织中的蛋白水平通常紧紧蛋白质生产和清除的平衡调节。使用荧光衰减光转化(FDAP)后,蛋白质的间隙动力学可以通过实验在体内测量。

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