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Mess Protein Stabilität in Wohnzebrafischembryonen unter Einsatz von Fluoreszenz Decay Nach Photokonversion (FDAP)
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP)
DOI:

09:45 min

January 28, 2015

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Chapters

  • 00:05Title
  • 01:27Generating a Photoconvertible Fusion Construct and Injecting Dechorionated Zebrafish Embryos
  • 03:22Mounting Zebrafish Embryos for Photoconversion and Confocal Imaging
  • 04:37Photoconverting and Measuring the Decrease of the Photoconverted Signal
  • 06:47Analyzing the Data Using PyFDAP
  • 08:18Results: Half-life of Squint-Dendra2 as Determined by Fluorescence Decay After Photoconversion during Embryogenesis
  • 09:19Conclusion

Summary

Automatic Translation

Protein-Spiegel in Zellen und Geweben werden oft dicht durch das Gleichgewicht der Proteinproduktion und Freigabe geregelt. Verwendung Fluoreszenzabklingzeit Nach Photokonversion (FDAP) können die Clearance-Kinetik von Proteinen experimentell in vivo gemessen werden.

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