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Using Adeno-associated Virus as a Tool to Study Retinal Barriers in Disease
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Using Adeno-associated Virus as a Tool to Study Retinal Barriers in Disease

Using Adeno-associated Virus as a Tool to Study Retinal Barriers in Disease

English

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14,906 Views

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10:14 min

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April 19, 2015

DOI:

10.3791/52451-v

DOI:
10.3791/52451-v

•

10:14 min
April 19, 2015

•

9 Views
, , , , ,
1Department of Therapeutics, Institut de la Vision,Sorbonne Universtés, UPMC Univ Paris 06, UMR_S 968, 2INSERM, U968, 3CNRS, UMR_7210

Summary

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To investigate the blood-retinal barrier permeability and the inner limiting membrane integrity in animal models of retinal disease, we used several adeno-associated virus (AAV) variants as tools to label retinal neurons and glia. Virus mediated reporter gene expression is then used as an indicator of retinal barrier permeability.

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Cite this Article

Vacca, O., El Mathari, B., Darche, M., Sahel, J., Rendon, A., Dalkara, D. Using Adeno-associated Virus as a Tool to Study Retinal Barriers in Disease. J. Vis. Exp. (98), e52451, doi:10.3791/52451 (2015).

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