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Using Adeno-associated Virus as a Tool to Study Retinal Barriers in Disease
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JoVE Journal Medicine
Using Adeno-associated Virus as a Tool to Study Retinal Barriers in Disease

Using Adeno-associated Virus as a Tool to Study Retinal Barriers in Disease

DOI:
10.3791/52451-v

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10:14 min

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April 19, 2015

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, , , , ,
1Department of Therapeutics, Institut de la Vision,Sorbonne Universtés, UPMC Univ Paris 06, UMR_S 968, 2INSERM, U968, 3CNRS, UMR_7210

Chapters

  • 00:05Title
  • 02:01Intravitreal Injection of AAV
  • 03:52Immunohistochemistry: Retinal Cryosections
  • 05:04Immunohistochemistry: Retinal Flatmounts
  • 06:15Immunohistochemistry: Labeling, Mounting, and Imaging
  • 07:24PCR Analysis of Mouse Blood Samples
  • 08:24Results: Retinal Permeability in Dp71-null Mice
  • 09:40Conclusion

Summary

Automatic Translation

Automatic Translation

To investigate the blood-retinal barrier permeability and the inner limiting membrane integrity in animal models of retinal disease, we used several adeno-associated virus (AAV) variants as tools to label retinal neurons and glia. Virus mediated reporter gene expression is then used as an indicator of retinal barrier permeability.

Tags

Keywords: Adeno-associated VirusRetinal BarriersMüller CellsBlood-retinal BarrierInner Limiting MembraneAAV5ShH10Retinal DiseaseGene DeliveryPermeability

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