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Mosaic Zebrafish Transgénese para Análise Funcional Genomic de candidatos Genes de cooperação em Tumor Patogênese
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Mosaic Zebrafish Transgenesis for Functional Genomic Analysis of Candidate Cooperative Genes in Tumor Pathogenesis
DOI:

09:45 min

March 31, 2015

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Chapters

  • 00:05Title
  • 01:21DNA Constructs and Transgenesis
  • 04:10Microinjection
  • 05:24Screen for Stable or Mosaic Transgenic Fish
  • 07:40Results: ALK and MYCN Induced Neuroblastoma Initiation
  • 09:13Conclusion

Summary

Automatic Translation

The goal of this study is to demonstrate how the mosaic transgenesis strategy can be used in zebrafish to rapidly and efficiently assess the relative contributions of multiple oncogenes in tumor initiation and progression in vivo.

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