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Probe-baseret real-time PCR metoder til kvantitativ måling af microRNA
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Biology
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JoVE Journal Biology
Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Probe-baseret real-time PCR metoder til kvantitativ måling af microRNA

DOI:
10.3791/52586-v

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10:28 min

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April 14, 2015

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, , , ,
1Diabetes and Islet Biology Group, NHMRC Clinical Trials Centre, Faculty of Medicine,The University of Sydney, 2Biomarkers Laboratory, NHMRC Clinical Trials Centre, Faculty of Medicine,The University of Sydney

Chapters

  • 00:05Title
  • 01:29Probe-based RTqPCR Mature MicroRNA (miRNA) Detection and Microfluidics Array Card Preparation
  • 03:40Microfluidics Card Loading and qPCR
  • 04:59Nanofluidics Array Loading and qPCR
  • 08:05Results: Representative miRNA Profile
  • 09:38Conclusion

Summary

Automatic Translation

Automatic Translation

Cirkulerende microRNA har for nylig vist sig som lovende og nye biomarkører for forskellige kræftformer og andre sygdomme. Målet med denne artikel er at diskutere tre forskellige probe-baseret real-time PCR-platforme og metoder, der er til rådighed til at kvantificere og bestemme den overflod af cirkulerende microRNA.

Tags

Probe-based QPCRMicroRNA QuantificationTranscript AbundanceHigh-throughput PlatformsMicrofluidicsNanofluidics ArraysTaqMan Array CardOpenArraySerumPlasma-EDTA

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