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三维组织工程人食管黏膜型号生产,表征和潜在用途
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Bioengineering
Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model
Please note that all translations are automatically generated.
Click here for the English version.
三维组织工程人食管黏膜型号生产,表征和潜在用途
DOI:
10.3791/52693-v
•
12:16 min
•
May 18, 2015
•
Nicola H. Green
,
Bernard M. Corfe
,
Jonathan P. Bury
,
Sheila MacNeil
1
Department of Materials Science and Engineering
,
University of Sheffield
,
2
Department of Oncology and Insigneo Institute for in silico Medicine
,
University of Sheffield
,
3
Department of Histopathology
,
Sheffield Teaching Hospitals NHS Foundation Trust
Chapters
00:05
Title
02:05
Human Esophageal Epithelial Cell and Fibroblast Isolation
04:08
De-cellularized Pig Esophageal Scaffold Preparation
06:27
Human Esophageal Mucosa Model Preparation
09:44
Results: Representative 3D Tissue-engineered Human Esophageal Mucosa
11:26
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这份手稿描述了生产,表征和组织工程食管三维结构从正常原代人成纤维食管,并在脱细胞化猪接种支架鳞状上皮细胞制备的潜在用途。结果证明类似于正常人食道成熟复层上皮的形成。
Tags
Esophageal Mucosa
Esophageal Adenocarcinoma
Barrett’s Metaplasia
Esophageal Fibroblasts
Esophageal Epithelial Cells
Acellular Esophageal Scaffold
Immunohistochemical Characterization
CK4
CK14
Ki67
Involucrin
Biologically Relevant Experimental Model
Pharmacological Agents
Environmental Factors
Alcohol
Toxins
High Temperature
Gastro-esophageal Reflux
Extended Culture
Esophageal Tumor Cell Lines
Barrett’s Cell Lines
Tumor Invasion
Drug Responsiveness
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