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三维组织工程人食管黏膜型号生产，表征和潜在用途
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Bioengineering
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JoVE Journal Bioengineering
Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model
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三维组织工程人食管黏膜型号生产，表征和潜在用途

DOI:
10.3791/52693-v

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12:16 min

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May 18, 2015

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, , ,
1Department of Materials Science and Engineering,University of Sheffield, 2Department of Oncology and Insigneo Institute for in silico Medicine,University of Sheffield, 3Department of Histopathology,Sheffield Teaching Hospitals NHS Foundation Trust

Chapters

  • 00:05Title
  • 02:05Human Esophageal Epithelial Cell and Fibroblast Isolation
  • 04:08De-cellularized Pig Esophageal Scaffold Preparation
  • 06:27Human Esophageal Mucosa Model Preparation
  • 09:44Results: Representative 3D Tissue-engineered Human Esophageal Mucosa
  • 11:26Conclusion

Summary

Automatic Translation

Automatic Translation

这份手稿描述了生产，表征和组织工程食管三维结构从正常原代人成纤维食管，并在脱细胞化猪接种支架鳞状上皮细胞制备的潜在用途。结果证明类似于正常人食道成熟复层上皮的形成。

Tags

Esophageal MucosaEsophageal AdenocarcinomaBarrett’s MetaplasiaEsophageal FibroblastsEsophageal Epithelial CellsAcellular Esophageal ScaffoldImmunohistochemical CharacterizationCK4CK14Ki67InvolucrinBiologically Relevant Experimental ModelPharmacological AgentsEnvironmental FactorsAlcoholToxinsHigh TemperatureGastro-esophageal RefluxExtended CultureEsophageal Tumor Cell LinesBarrett’s Cell LinesTumor InvasionDrug Responsiveness

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