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In vivo Dynamics of Retinal mikrogliaceller Aktivering Under Neurodegeneration: Konfokal oftalmoskopiske Imaging og Cell Morfometri i Mouse Glaukom
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JoVE Journal Medicine
In Vivo Dynamics of Retinal Microglial Activation During Neurodegeneration: Confocal Ophthalmoscopic Imaging and Cell Morphometry in Mouse Glaucoma

In vivo Dynamics of Retinal mikrogliaceller Aktivering Under Neurodegeneration: Konfokal oftalmoskopiske Imaging og Cell Morfometri i Mouse Glaukom

DOI:
10.3791/52731-v

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12:48 min

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May 11, 2015

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, , ,
1Department of Neurobiology & Anatomy,University of Utah, 2Department of Ophthalmology & Visual Sciences,University of Utah

Chapters

  • 00:05Title
  • 01:55Setting Up for Confocal Scanning Laser Ophthalmoscopy (cSLO)
  • 02:57In Vivo Imaging of Retinal Microglia by cSLO
  • 06:26Live Image Processing and Microglial Cell Segmentation
  • 08:19Live Image Analysis and Microglial Morphometry
  • 10:22Results: cSLO Imaging of Retinal Microglia Cells
  • 11:41Conclusion

Summary

Automatic Translation

Automatic Translation

Mikroglia aktivering og mikrogliose er centrale reaktioner på kronisk neurodegeneration. Her præsenterer vi metoder til in vivo, langsigtet visualisering af retinale CX3CR1-GFP + mikrogliaceller celler ved konfokal oftalmoskopi og til grænsen og morfometrisk analyser at identificere og kvantificere deres aktivering. Vi overvåger mikrogliaceller ændringer i løbet tidlige stadier af aldersrelateret glaukom.

Tags

Retinal MicrogliaIn Vivo ImagingConfocal OphthalmoscopyCX3CR1GFP/+ Reporter MiceMicroglial ActivationMicroglial MorphometryChronic NeurodegenerationGlaucomaRetinal NeurodegenerationOptic Nerve

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