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视网膜胶质细胞活化的体内动力学在神经退行性疾病：共聚焦成像眼底及细胞形态计量小鼠青光眼
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JoVE Journal Medicine
In Vivo Dynamics of Retinal Microglial Activation During Neurodegeneration: Confocal Ophthalmoscopic Imaging and Cell Morphometry in Mouse Glaucoma
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视网膜胶质细胞活化的体内动力学在神经退行性疾病：共聚焦成像眼底及细胞形态计量小鼠青光眼

DOI:
10.3791/52731-v

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12:48 min

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May 11, 2015

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, , ,
1Department of Neurobiology & Anatomy,University of Utah, 2Department of Ophthalmology & Visual Sciences,University of Utah

Chapters

  • 00:05Title
  • 01:55Setting Up for Confocal Scanning Laser Ophthalmoscopy (cSLO)
  • 02:57In Vivo Imaging of Retinal Microglia by cSLO
  • 06:26Live Image Processing and Microglial Cell Segmentation
  • 08:19Live Image Analysis and Microglial Morphometry
  • 10:22Results: cSLO Imaging of Retinal Microglia Cells
  • 11:41Conclusion

Summary

Automatic Translation

Automatic Translation

小胶质细胞活化和小神经胶质细胞是关键应对慢性神经退行性病变。这里，我们提出在体内 ，视网膜CX3CR1-GFP +胶质细胞共聚焦检眼镜的长期可视化方法，以及用于阈和形态分析，以确定和量化它们的活化。我们监测期间与年龄有关的青光眼的早期阶段小胶质细胞的变化。

Tags

Retinal MicrogliaIn Vivo ImagingConfocal OphthalmoscopyCX3CR1GFP/+ Reporter MiceMicroglial ActivationMicroglial MorphometryChronic NeurodegenerationGlaucomaRetinal NeurodegenerationOptic Nerve

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