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视网膜胶质细胞活化的体内动力学在神经退行性疾病:共聚焦成像眼底及细胞形态计量小鼠青光眼
JoVE Journal
Medicine
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JoVE Journal Medicine
In Vivo Dynamics of Retinal Microglial Activation During Neurodegeneration: Confocal Ophthalmoscopic Imaging and Cell Morphometry in Mouse Glaucoma
DOI:

12:48 min

May 11, 2015

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Chapters

  • 00:05Title
  • 01:55Setting Up for Confocal Scanning Laser Ophthalmoscopy (cSLO)
  • 02:57In Vivo Imaging of Retinal Microglia by cSLO
  • 06:26Live Image Processing and Microglial Cell Segmentation
  • 08:19Live Image Analysis and Microglial Morphometry
  • 10:22Results: cSLO Imaging of Retinal Microglia Cells
  • 11:41Conclusion

Summary

Automatic Translation

小胶质细胞活化和小神经胶质细胞是关键应对慢性神经退行性病变。这里,我们提出在体内 ,视网膜CX3CR1-GFP +胶质细胞共聚焦检眼镜的长期可视化方法,以及用于阈和形态分析,以确定和量化它们的活化。我们监测期间与年龄有关的青光眼的早期阶段小胶质细胞的变化。

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