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August 16, 2015
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Summary
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يصف هذا البروتوكول استخدام bromodeoxyuridine (BrdU) امتصاص للسماح تتبع الزمني للخلايا التي كانت في المرحلة S في نقطة محددة في الوقت المناسب. إضافة الأصباغ الحمض النووي ووضع العلامات الأجسام المضادة تسهل تحليل مفصل لمصير الخلايا مرحلة S في أوقات لاحقة.
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Cite this Article
Welschinger, R., Bendall, L. J. Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization. J. Vis. Exp. (102), e52840, doi:10.3791/52840 (2015).
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