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08:52 min
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August 16, 2015
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Summary
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Questo protocollo descrive l'uso di bromodeossiuridina (BrdU) uptake per consentire il monitoraggio temporale di cellule che erano in fase S in un determinato momento. L'aggiunta di coloranti DNA e l'etichettatura degli anticorpi facilita l'analisi dettagliata del destino delle cellule in fase S in tempi successivi.
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Cite this Article
Welschinger, R., Bendall, L. J. Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization. J. Vis. Exp. (102), e52840, doi:10.3791/52840 (2015).
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