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JoVE Journal
Immunology and Infection

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Isolation and Characterization of Neutrophil-derived Microparticles for Functional Studies
 

Isolation and Characterization of Neutrophil-derived Microparticles for Functional Studies

Article DOI: 10.3791/56949-v 06:56 min March 2nd, 2018
March 2nd, 2018

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Summary

New protocols are described here to isolate and characterize microparticles derived from human and mouse neutrophils. These protocols utilize ultracentrifugation, flow cytometry, and immunoblotting techniques to analyze microparticle content, and they can be used to study the role of microparticles derived from various cell types in cellular function.

Tags

Keywords: Neutrophil-derived Microparticles Cell-cell Communication Density Gradient Separation Polymorphonuclear Cells PMN Mononuclear Cells Cell-free Supernatants Ultra-centrifugation Functional Studies
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