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Cryosectioning Method for Microdissection of Murine Colonic Mucosa
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Cryosectioning Method for Microdissection of Murine Colonic Mucosa

Cryosectioning Method for Microdissection of Murine Colonic Mucosa

DOI:
10.3791/53112-v

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06:16 min

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July 12, 2015

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, , , ,
1Epithelial Pathobiology and Mucosal Inflammation Research Unit, Department of Pathology and Laboratory Medicine,Emory University, Atlanta, Georgia

Chapters

  • 00:05Title
  • 01:07Preparation for Cryosectioning
  • 02:04Dissection of Distal Colonic Tissue
  • 02:51Mounting and Cryosectioning the Tissue
  • 04:15Results: Morphology of Sectioned Tissue
  • 05:55Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a simple method for the isolation of spatially distinct murine colonic epithelial surface cells from the underlying crypt-base cells.

Tags

Keywords: CryosectioningMicrodissectionMurineColonic MucosaEpithelial CellsCrypts Of LieberkuhnProgenitor CellsCellular DifferentiationProliferative Crypt ZoneSurface Epithelial CellsRNA IsolationProtein Isolation

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