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Tre-dimensionelle billedbehandling og analyse af mitochondrier i menneskelige Intraepidermal nervefibre
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Neuroscience
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JoVE Journal Neuroscience
Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers

Tre-dimensionelle billedbehandling og analyse af mitochondrier i menneskelige Intraepidermal nervefibre

DOI:
10.3791/53369-v

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10:31 min

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September 29, 2017

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, , ,
1University of Michigan Medical School, 2Department of Neurology,University of Michigan, 3Department of Internal Medicine,University of Michigan

Chapters

  • 00:05Title
  • 01:02Fluorescence Immunohistochemistry: Day 1
  • 02:27Fluorescence Immunohistochemistry: Day 2
  • 03:21Fluorescence Immunohistochemistry: Day 3
  • 04:22Confocal Imaging
  • 06:00Image Processing
  • 07:03Isolation of Nerve-specific Mitochondrial Signal
  • 08:41Results: Analysis of Mitochondria within Human Intraepidermal Nerve Fibers
  • 09:39Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol bruger tre-dimensionelle (3D) billedbehandling og analyse teknikker til at visualisere og kvantificere nerve-specifikke mitokondrier. Teknikkerne, der er gældende for andre situationer, hvor en fluorescerende signal bruges til at isolere et undersæt af data fra en anden fluorescerende signal.

Tags

3D ImagingMitochondriaIntraepidermal Nerve FibersFluorescence ImmunohistochemistryPGP9.5PDHSecondary AntibodiesSignal EnhancementEpidermal Nerve FibersNeurological Complications

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