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Chapters
- 00:05Title
- 00:39Isolation of Flexor Digitorum Brevis (FDB) Fibers
- 03:12Dissociating the FDB Muscle Fibers for Imaging
- 05:48Results: FM-dye Uptake by Isolated Myofibers Expressing Fluorescently Tagged Proteins
- 07:24Conclusion
This protocol utilizes electroporation to introduce and express fluorescently labeled proteins in mouse muscle fibers. Following recovery after electroporation, fibers are isolated. Individual fibers are then imaged using high resolution confocal microscopy to visualize muscle structure.
