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高密度条码抗体芯片的水流模式的指导下制作
JoVE Journal
Chemistry
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JoVE Journal
Chemistry
Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray
Please note that all translations are automatically generated.
Click here for the English version.
高密度条码抗体芯片的水流模式的指导下制作
DOI:
10.3791/53644-v
•
09:05 min
•
January 06, 2016
•
Lisa S. Ramirez
2
,
Jun Wang
2
1
Department of Chemistry
,
University at Albany, State University of New York
,
2
Multiplex Biotechnology Laboratory
,
Cancer Research Center
Chapters
00:05
Title
00:44
One-dimensional DNA Barcode Slides: Preparing the PDMS Barcode Mold
02:21
One-dimensional DNA Barcode Slides: Patterning of Poly-L-lysine (PLL)
03:39
One-dimensional DNA Barcode Slides: Patterning of ssDNA
05:00
Two-dimensional 3×3 DNA Arrays: Preparing the PDMS Barcode Mold and Flow Patterning of ssDNA
06:17
Two-dimensional 3×3 DNA Arrays: Validation and Immunoassay
07:32
Results: Multiplex-protein Detection from Single-cell Preparations
08:25
Conclusion
Summary
Automatic Translation
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Automatic Translation
这个协议概括了大规模的制造中,复用的二维DNA或抗体阵列,在细胞信号研究和生物标记物检测的应用潜力。
Tags
Flow-pattern
Barcode
Antibody Microarray
Single-cell Analysis
PDMS Mold
SU-8 Master
Photo-mask
Poly-L-lysine
Polyethylene Tubing
Stainless Steel Pins
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