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Technical Aspect of the Automated Synthesis and Real-Time Kinetic Evaluation of [11C]SNAP-7941
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Chemistry
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JoVE Journal Chemistry
Technical Aspect of the Automated Synthesis and Real-Time Kinetic Evaluation of [11C]SNAP-7941

Technical Aspect of the Automated Synthesis and Real-Time Kinetic Evaluation of [11C]SNAP-7941

DOI:
10.3791/59557-v

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09:50 min

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April 28, 2019

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, , , , , , ,
1Department of Biomedical Imaging and Image-guided Therapy, Division of Nuclear Medicine,Medical University of Vienna, 2Department for Pharmaceutical Technology and Biopharmaceutics, Faculty of Life Sciences,University of Vienna, 3Ludwig Boltzmann Institute Applied Diagnostics, 4CBmed GmbH – Center for Biomarker Research in Medicine

Chapters

  • 00:04Title
  • 01:20Automated Synthesis of [11C]SNAP-7941 for Preclinical Use
  • 04:40Quality Control (QC) of [11C]SNAP-7941
  • 05:55Real-Time Kinetic Assay
  • 08:02Results: Evaluation of [11C]SNAP-7941 Interactions Towards the Permeability Glycoprotein 1 (P-gp) Transporter
  • 08:44Conclusion

Summary

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Here, we represent a protocol for the fully automated radiolabelling of [11C]SNAP-7941 and the analysis of the real-time kinetics of this PET-tracer on P-gp expressing and non-expressing cells.

Tags

Keywords: [11C]SNAP-7941Carbon-11 RadiolabelingPETMelanin-concentrating Hormone ReceptorEfflux TransportersAutomated RadiosynthesisMethyl Group

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