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损失 - 和增益的功能角度进行调查研究早期细胞命运决定在植入前小鼠胚胎
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos
DOI:

08:43 min

June 06, 2016

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Chapters

  • 00:05Title
  • 01:13Oviduct Isolation
  • 02:17Retrieval and Culturing of 2-Pronuclear (2-PN) Embryos
  • 03:46Microinjecting 2-PN Embryos
  • 06:29Results: Neogenin Expression Manipulations
  • 07:59Conclusion

Summary

Automatic Translation

该协议的目的是描述一个损失 - 和增益的是适用于确定neogenin作为通向滋养外胚层和中植入前小鼠胚胎内细胞团的分化阶段特异性受体功能的方法。

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