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Developmental Biology
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损失 - 和增益的功能角度进行调查研究早期细胞命运决定在植入前小鼠胚胎
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos
Please note that all translations are automatically generated.
Click here for the English version.
损失 - 和增益的功能角度进行调查研究早期细胞命运决定在植入前小鼠胚胎
DOI:
10.3791/53696-v
•
08:43 min
•
June 06, 2016
•
Jae H. Lee
2
,
Yong II Cho
,
Sung S. Choi
,
Hae-Won Kim
2
,
Churl K. Min
,
Sang J. Lee
1
Department of Nanobiomedical Sciences and BK21 PLUS NBM Global Research Center for Regenerative Medicine
,
Dankook University
,
2
Insititute of Tissue Regeneration Engineering
,
Dankook University
,
3
Department of Biological Sciences
,
Ajou University
,
4
Department of Pharmacy
,
Sahmyook University
,
5
Department of Animal Biotechnology
,
Sahmyook University
Chapters
00:05
Title
01:13
Oviduct Isolation
02:17
Retrieval and Culturing of 2-Pronuclear (2-PN) Embryos
03:46
Microinjecting 2-PN Embryos
06:29
Results: Neogenin Expression Manipulations
07:59
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议的目的是描述一个损失 - 和增益的是适用于确定neogenin作为通向滋养外胚层和中植入前小鼠胚胎内细胞团的分化阶段特异性受体功能的方法。
Tags
Loss-of-function
Gain-of-function
Cell Fate
Preimplantation
Mouse Embryos
Inner Cell Mass
Differentiation
Stage-specific
Embryonic Stem Cell
Receptor
Ligand
Developmental Biology
Stem Cell Biology
Animal Science
Reproductive Biotechnology
Animal Cloning
2-PN Embryos
Cumulus Cells
Hyaluronidase
Denuding
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